The lab uses a well-characterized in vitro model of the central nervous system, the hippocampal slice preparation. Electrophysiological recordings from neurons are utilized to monitor the effects of various drugs applied to the tissue, as well as the effects of neurotransmitters released from endogenous sources within the slice.

Basic properties of synaptic transmission and neuron excitability are evaluated before and following exposure to various compounds in order to investigate the mechanisms underlying the effects of these drugs. In addition, two proposed models for learning and memory processes at the cellular level, Long-term Potentiation and Long-term Depression, are evoked in order to evaluate the potential effects of drugs of abuse on hippocampal function. In addition to acute, in vitro drug application directly onto the hippocampal slice, we also obtain tissue from animals allowed to self-administer drugs such as cocaine and morphine.

Through investigating how exposure to drugs of abuse impacts hippocampal function at the synaptic level, we will determine molecular and cellular mechanisms by which such compounds may alter neuronal function. These changes are likely to be representative of those that underlie the initiation and maintenance of the addicted state in susceptible individuals.