The University of Georgia College of Veterinary Medicine

Monoclonal Antibody Facility

Introduction

The Monoclonal Antibody Facility of the University of Georgia is available for any investigator who has a need for its services. Located in the College of Veterinary Medicine, the facility is staffed by five full-time personnel and is supported in part by the College's Animal Resources unit.

The facility is equipped to immunize and monitor mice, perform fusions of mouse spleen cells and myelomas to generate antibody-producing hybridomas, isolate and propagate selected cell clones, preserve these cell lines in liquid nitrogen, and produce quantities of antibody. The facility can produce antibodies to synthetic peptides, haptens coupled to carrier proteins, fusion proteins, and more conventional antigens.

The hybridomas resulting from the fusions are screened for antibody production by ELISA. Then, the selected hybridomas are propagated in tissue culture flasks by in vitro techniques rather than using additional mice to produce ascites fluid.

To arrange for use of the facility, or for further information, please contact Ruth Davis (e-mail rhd@uga.edu) at 706.542.1848. You may also visit the facility at Room 1112A, in the College of Veterinary Medicine.

This page was last updated on February 9, 2009.