Viral Immunology: Tripp Laboratory
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- Antibody Prep and Hybridomas
- Buffers and Stocks
- Cell Prep
- Cell Staining/Analysis
- Conjugations
- EIAs
- ELISAs
- ELISPOTs
- Functional Assays
- HMPV
- IHC
- Influenza
- Molecular
- Nanotech
- Plaque Assays
- Purification
- SARS
- siRNA
- Virus Prep
- Westerns
Laboratory Protocols
Antibody Preparation & Hybridomas
- Purification of Monoclonal Antibodies -GL-Ab-1
- Ammonium Sulfate Precipitation of Antibodies
- Digesting Antibodies with Pepsin to Yield F(ab)’ 2 Antibody Fragments
- Preparing Antisera to Peptides
- General Immunoprecipitation
- The Basics of Hybridoma Cell Culture from Start to Freeze
- Hybridoma Media Preparation: DMEM-10
- Hybridoma Media Preparation: SP2/0-conditioned Medium
- Hybridoma Media Preparation: 50X HAT
- Hybridoma Media Preparation: DMEM 50/10
- Hybridoma Media Preparation: MRA, 8-Aza, 50/10 DMEM
- Hybridoma Media Preparation: DMEM 50/10 HAT
- Hybridoma Media Preparation: DMEM 50/10 HT
- Hybridoma Media Preparation: Freezing Medium
- Hybridoma Generation: Animal Boosting
- Hybridoma Generation: Adenovirus (Ad)Expression vector-spike (Ad-S) or Ad-nucleoprotein (Ad-N)
- Hybridoma Generation: Sera Screening
- Hybridoma Generation: Inactivated Gradient-Enriched Coronavirus (CV)
- Hybridoma Generation: Cell Fusion of myeloma SP2/0
Buffers and Stock Solutions
- 2-Mercaptoethanol (2-ME), 50mM
- Ammonium Acetate, 10 M
- Avertin Stock Solution
- Alsever’s solution
- Carbonate Buffer
- Denhardt Solution
- Dithiothreitol (DTT), 1M
- Ethylenediaminetetraacetic Acid (EDTA), 0.5M pH 8.0
- Hanks’ Balanced SaltSolution (HBSS)
- Hydrochloric Acid (HCL), 1M
- MgCl2, 1M
- NaOH, 10M
- Potassium Phosphate Buffer, 0.1 M
- Paraformaldehyde Fixation Buffer (PFA)
- Sodium Dodecyl Sulfate or Sodium Lauryl Sulfate (SDS), 20% (w/v)
- Tris-Buffered Saline (TBS); Tris/EDTA Buffer (TE); Tris-Cl, 1M
- Tumor Cocktail Medium
Cell Preparation
- Bovine Pituitary Extract (BPE) Preparation
- Collection of Bronchoalveolar (BAL) Cells
- Collection of Total Lung Cells
- Collection of murine peritoneal macrophages
- Counting Cells
- Eliciting Peritoneal Macrophages with ConA
- Freezing and Thawing Cell Lines
- Isolation of Murine Bone Marrow Macrophages
- Lung Digestion:Collagenase/Dnase
- Normal Human Bronchial Epithelial Cells (NHBE) Air/Liquid Interface Culture System -GL-CP14
- Normal Human Bronchial Epithelial Cell: (NHBE) Grow-up media -GL-CP15
- Normal Human Bronchial Epithelial Cell: (NHBE) 50.50 Media -GL-CP16
- Normal Human Bronchial Epithelial Cell: Collagen Thin Coating Prep -GL-CP17
- Nonionic Detergent Solubilization of Lymphocytes
- Passaging Vero Cells: Trypsin Protocol
- Preparing Bone Marrow Macrophages for use as Antigen Presenting Cells: Interferon Gamma (IFN-g)
- Preparing Lipopolysaccharide (LPS)-stimulatedB Lymphoblasts for use as Antigen-Presenting Cells
Cell Staining/Analysis
Conjugations
EIAs
ELISAs
- Basic Enzyme-Linked Immunosorbent Assay (ELISA)
- ELISA to Detect anti-Substance P Antibody
- aMPV-specific Enzyme-Linked Immunosorbent Assay (ELISA)
- hMPV detection by ELISA -GL-EL-4
- Enzyme-Linked Immunosorbent Assay (ELISA) for Mouse EPO Activity
- Measurement of IgG, IgM, and IgA Responses by ELISA
- RSV Glycoprotein-G ELISA
- RSV ELISA
- Tissue Culture ELISA for RSV
- ELISA: WNV Envelope-Peptide
- siRNA Transfection/Viral Infection-ELISA
ELISPOTs
Functional Assays
- ADCC and NK Cell Assays of BAL Cells
- Characterization of Intracellular Cytokine Response to Overlapping RSV G Peptide
- CTL Re-stimulation and Chromium Release Assay -GL-FA-3
- CTL Assay: IFN-gamma ELISPOT -GL-FA-4
- CTL Functional Assay for Flow Cytometry
- Cell Migration Studies
- Natural Killer Cell Functional Assay for Flow Cytometry
- DiOC18/Propidium Iodide (PI) Natural Killer (NK) Cell Assay
- Non-radioactive Cytolytic Assay
- Nitrite (NO) Determination: A Modified Greiss Reaction
- T Cell Proliferation Assay: MHC II Peptides
- TNF Assay
- CTL Secondary Restimulation Assay for BALB/c Mice (H-2d)
Human Metapneumovirus
Immunohistochemistry
- Immunostaining for Respiratory Syncytial Virus (RSV) -GL-IH-1
- Immunostaining for Respiratory Syncytial Virus (RSV):LabTek Slide Format
- Immunostaining for Human Meta-pneumovirus (hMPV) -GL-IH-4
- Immunohistochemistry (IHC) for LabTek Slides
- Basic Immunohistochemistry for Parafin- and Formalin-Fixed Tissues
- Immunohistochemistry (IHC) for Avian Metapneumovirus (aMPV)
- IHC: Immunoperoxidase Staining
Influenza
Molecular
Nanotechnology
Plaque Assays
Purification
SARS Coronavirus
siRNA
Virus Preparation
- BPL-inactivation of Ampv
- Propagation of RSV: Vero Cell Culture
- Propagation of RSV: Hep-2 Cell Culture
- Human Metapneumovirus (hMPV) Culture -GL-VP-4
- Human metapneumovirus (hMPV) Culture Medium -GL-VP-5
Western Blots
This page last updated May 7, 2008 .
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