Viral Immunology:Tripp Laboratory
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Ralph Tripp's Quicklinks:
UGA Faculty of Infectious Diseases
Nanoscale Science and Engineering Center (NanoSEC)
Georgia Research Alliance
Ralph A. Tripp, PhD
Professor and Georgia Research Alliance Eminent Scholar
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Research Interests
The research interest of our group is to develop translational disease intervention strategies for important human viruses and emerging infectious diseases of zoonotic origin. Our BSL2, BSL3 and BSL3Ag+ laboratories develop platform enabling technologies in pathogen biosensing using nanotechnology-based approaches, antiviral drugs using small molecule and RNAi-based drugs, and animal and human vaccines using state-of-the-art technologies with our in-house pilot GMP vaccine facility. In parallel, we investigate the mechanisms of immunity and disease pathogenesis associated with infection to better understand the conceptual and functional differences between innate and adaptive immune responses that provide the foundation necessary to facilitate vaccine and antiviral therapeutic protocols. The laboratories leverage the talents of academic, government and industry partners to promote ‘bench-to-bedside’ and ‘bench-to-barnyard’ vaccines and therapeutics.
Vision
To understand the dynamics of the host response to infection that will provide new strategies for resolving disease through the development of cutting edge treatments or vaccines. These studies will facilitate training of new scientists and scientists with diverse backgrounds who will be able to communicate their findings to industry, the public, and the academic community, to help make Georgia the preeminent center for vaccine research and development.
In the Lab
The fundamental goals of the laboratory are to facilitate disease intervention strategies that control or prevent important human viruses and emerging infectious diseases (EID) of zoonotic origin that pose significant health risks to human and/or animal hosts. The specific aims of the lab are to: 1) Develop a discovery-to-control continuum for the development and utilization of new and improved therapeutics, diagnostics and vaccines against these classes of pathogens, 2) Facilitate the interaction and collaboration of investigators and industry working on related diseases allowing for the cross-fertilization of ideas and multi-disciplinary approaches, 3) Develop national and international collaborations that allow for scientific exchanges and leveraging of talents, and 4) Promote the funding of these research efforts through governmental support, and develop industrial support for projects of commercial interest and/or the commercialization of products of research conducted in the lab.
Recent Publications
- Oshansky CM, Barber JP, Crabtree J,and Tripp R. Respiratory Syncytial Virus (RSV) F and G Proteins Induce IL-1 alpha, CC and CXC Chemokine Responses by Normal Human Bronchoepithelial Cells. J Infect Dis. 2010 Mar 5. [Epub ahead of print]
- Zhang W, Choi Y, Haynes LM, Harcourt JL, Anderson LJ, Jones LP, and Tripp RA. Vaccination to induce antibodies blocking the CX3C-CX3CR1 interaction of respiratory syncytial virus G protein reduces pulmonary inflammation and virus replication in mice. J Virol. 2010 Jan;84(2):1148-57. Epub 2009 Oct 28.
- Driskell JD, Tripp RA. Emerging Technologies in Nanotechnology-based Pathogen Detection. Clinical Microbiology Newsletter 2009;31(18):137.
- Driskell JD, Primera-Pedrozo OM, Dluhy RA, Zhao Y, and Tripp RA. Quantitative surface-enhanced Raman spectroscopy based analysis of microRNA mixtures. Appl Spectrosc. 2009 Oct;63(10):1107-14.
- Collarini EJ, Lee FE, Foord O, Park M, Sperinde G, Wu H, Harriman WD, Carroll SF, Ellsworth SL, Anderson LJ, Tripp RA, Walsh EE, Keyt BA, and Kauvar LM. Potent high-affinity antibodies for treatment and prophylaxis of respiratory syncytial virus derived from B cells of infected patients. J Immunol. 2009 Nov 15;183(10):6338-45. Epub 2009 Oct 19.
- Tripp RA, and Tompkins SM. Animal models for evaluation of influenza vaccines. Curr Top Microbiol Immunol. 2009;333:397-412. Review.
- Mundt E, Gay L, Jones L, Saavedra G, Tompkins SM, and Tripp RA. Replication and pathogenesis associated with H5N1, H5N2, and H5N3 low-pathogenic avian influenza virus infection in chickens and ducks. Arch Virol. 2009;154(8):1241-8. Epub 2009 Jul 3.
- Abell JL, Driskell JD, Dluhy RA, Tripp RA, and Zhao YP. Fabrication and characterization of a multiwell array SERS chip with biological applications. Biosens Bioelectron. 2009 Aug 15;24(12):3663-70. Epub 2009 Jun 7.
- Haynes LM, Caidi H, Radu GU, Miao C, Harcourt JL, Tripp RA, and Anderson LJ. Therapeutic monoclonal antibody treatment targeting respiratory syncytial virus (RSV) G protein mediates viral clearance and reduces the pathogenesis of RSV infection in BALB/c mice. J Infect Dis. 2009 Aug 1;200(3):439-47.
- Alvarez R, Elbashir S, Borland T, Toudjarska I, Hadwiger P, John M, Roehl I, Morskaya SS, Martinello R, Kahn J, Van Ranst M, Tripp RA, DeVincenzo JP, Pandey R, Maier M, Nechev L, Manoharan M, Kotelianski V, and Meyers R. RNA interference-mediated silencing of the respiratory syncytial virus nucleocapsid defines a potent antiviral strategy. Antimicrob Agents Chemother. 2009 Sep;53(9):3952-62. Epub 2009 Jun 8.
- Tripp RA, and Tompkins SM. Therapeutic applications of RNAi for silencing virus replication. Methods Mol Biol. 2009;555:43-61.
- Oshansky CM, Krunkosky TM, Barber J, Jones LP, and Tripp RA. Respiratory syncytial virus proteins modulate suppressors of cytokine signaling 1 and 3 and the type I interferon response to infection by a toll-like receptor pathway. Viral Immunol. 2009 Jun;22(3):147-61.
- Liu Y, Haas DL, Poore S, Isakovic S, Gahan M, Mahalingam S, Fu ZF, and Tripp RA. Human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment. J Virol. 2009 Jul;83(13):6837-48. Epub 2009 Apr 8.
- Oshansky CM, Zhang W, Moore E, and Tripp RA. The host response and molecular pathogenesis associated with respiratory syncytial virus infection. Future Microbiol. 2009 Apr;4:279-97. Review.
- Miao C, Radu GU, Caidi H, Tripp RA, Anderson LJ, and Haynes LM. Treatment with respiratory syncytial virus G glycoprotein monoclonal antibody or F(ab')2 components mediates reduced pulmonary inflammation in mice. J Gen Virol. 2009 May;90(Pt 5):1119-23. Epub 2009 Mar 4.
- Zhang W, and Tripp RA. RNA interference inhibits respiratory syncytial virus replication and disease pathogenesis without inhibiting priming of the memory immune response. J Virol. 2008 Dec;82(24):12221-31. Epub 2008 Sep 25.
- Moore EC, Barber J, and Tripp RA. Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15). Virol J. 2008 Oct 13;5:116.
- Driskell JD, Seto AG, Jones LP, Jokela S, Dluhy RA, Zhao YP, and Tripp RA. Rapid microRNA (miRNA) detection and classification via surface-enhanced Raman spectroscopy (SERS). Biosens Bioelectron. 2008 Dec 1;24(4):923-8. Epub 2008 Aug 6.
- Tauro S, Su YC, Thomas S, Schwarze J, Matthaei KI, Townsend D, Simson L, Tripp RA, and Mahalingam S. Molecular and cellular mechanisms in the viral exacerbation of asthma. Microbes Infect. 2008 Jul;10(9):1014-23. Epub 2008 Aug 13. Review.
- Tripp RA, and Tompkins SM. Recombinant vaccines for influenza virus. Curr Opin Investig Drugs. 2008 Aug;9(8):836-45. Review.
- Driskell JD, Shanmukh S, Liu YJ, Hennigan S, Jones L, Zhao YP, Dluhy RA, Krause DC, and Tripp RA. Infectious agent detection with SERS-active silver nanorod arrays prepared by oblique angle deposition. IEEE Sens. J. 2008, 8(6), 863.
- Fu J, Jones LJ, Tripp RA, and Zhao YP. Au/Si hetero-nanorod-based biosensor for Salmonella detection. Nanotechnology. 2008, in press.
- Murawski MR, Bowen GN, Cerny AM, Anderson LJ, Haynes LM, Tripp RA, Kurt-Jones EA, and Finberg RW. RSV activates innate immunity through Toll Like Receptor 2. J Virol. 2009 Feb;83(3):1492-500. Epub 2008 Nov 19.
- Shanmukh S, Jones L , Zhao YP, Driskell JD, Tripp RA, and Dluhy RA. Identification and classification of respiratory syncytial virus (RSV) strains by surface enhanced Raman spectroscopy and multivariate statistical techniques. Anal. Bioanal. Chem. 2008 Mar;390(6):1551-5. Epub 2008 Jan 31.
- Tripp RA, and Tompkins SM. Perspectives on the immunobiology of influenza virus. Research Signpost: Chapters 1, 4, 9 and 14. March 2008, in press.
- Tripp RA, and Tompkins SM. Current Topics in Microbiology and Immunology: Vaccines for Pandemic Influenza. Springer Publications. March 2008, in press.
- Tripp RA, and Tompkins SM. Virally-Encoded MicroRNA (miRNA): Candidates for Gene Silencing. CRC Press. April 2008, in press.
- Tripp RA, and Tompkins SM. Therapeutic applications of RNAi for silencing virus replication. Humana Press. May 2008, in press.
- Zhao YP, and Tripp RA. Nanomaterials for the Life Sciences: Spherical and anisotropic silver nanomaterials in medical diagnosis. July 2008, in press.
- Zhao YP, Dluhy RA, and Tripp RA. Novel nanostructures for surface-enhanced Raman spectroscopy (SERS) biosensing. NanoToday. 2008, in press.
- Tompkins SM, Lin Y, Leser GP, Kramer KA, Haas DL, Howerth EW, Xu J, Kennett MJ, Durbin RK, Durbin JE, Tripp R, Lamb RA, He B. Recombinant parainfluenza virus 5 (PIV5) expressing the influenza A virus hemagglutinin provides immunity in mice to influenza A virus challenge. Virology. 2007 May 25;362(1):139-50. Epub 2007 Jan 23.
- Birkness KA, Guarner J, Sable SB, Tripp RA, Kellar KL, Bartlett J, and Quinn FD. An in vitro model of the leukocyte interactions associated with granuloma formation in Mycobacterium tuberculosis infection. Immunol Cell Biol. 2007 Feb-Mar;85(2):160-8. Epub 2007 Jan 2.
- Ribeiro LZ, Tripp RA, Rossi LM, Palma PV, Yokosawa J, Mantese OC, Oliveira TF, Nepomuceno LL, and Queiróz DA. Serum mannose-binding lectin levels are linked with respiratory syncytial virus (RSV) disease. J Clin Immunol. 2008 Mar;28(2):166-73. Epub 2007 Oct 20.
- Tripp RA, Alvarez R, Anderson B, Jones L, Weeks C, and Chen W. Bioconjugated nanoparticle detection of respiratory syncytial virus infection. Int J Nanomedicine. 2007;2(1):117-24.
- Shanmukh S, Jones L, Driskell J, Zhao Y, Dluhy R, and Tripp RA. Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate. Nano Lett. 2006 Nov;6(11):2630-6.
- Agrawal A, Zhang C, Byassee T, Tripp RA, and Nie S. Counting single native biomolecules and intact viruses with color-coded nanoparticles. Anal Chem. 2006 Feb 15;78(4):1061-70.
- Harcourt J, Alvarez R, Jones LP, Henderson C, Anderson LJ, and Tripp RA. Respiratory syncytial virus G protein and G protein CX3C motif adversely affect CX3CR1+ T cell responses. J Immunol. 2006 Feb 1;176(3):1600-8.
- Li XQ, Fu ZF, Alvarez R, Henderson C, and Tripp RA. Respiratory syncytial virus (RSV) infects neuronal cells and processes that innervate the lung by a process involving RSV G protein. J.Virol. 2006 Jan;80(1):537-40.
- Mahalingam S, and Tripp RA. Perspective on the host response to human metapneumovirus infection: what can we learn from respiratory syncytial virus infections? Microbes Infect. 2006 Jan;8(1):285-93. Epub 2005 Aug 10. Review.
- Shanmukh S, Jones L, Driskell J, Zhao Y, Dluhy R, and Tripp RA. Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate. Nano Lett. 2006 Nov;6(11):2630-6.
- Agrawal A, Tripp RA, Anderson LJ, and Nie S. Real-time detection of virus particles and viral protein expression with two-color nanoparticle probes. J Virol. 2005 Jul;79(13):8625-8.
- Alvarez R, and Tripp RA. The immune response to human metapneumovirus (HMPV) is associated with an aberrant immunity and imparied virus clearance in BALB/c mice. J. of Virol. 2005 May;79(10):5971-8.
- Tripp RA, Haynes LM, Moore D, Anderson B, Tamin A, Harcourt BH, Jones LP, Yilla M, Babcock GJ, Greenough T, Ambrosino DM, Alvarez R, Callaway J, Cavitt S, Kamrud K, Alterson H, Smith J, Harcourt JL, Miao C, Razdan R, Comer JA, Rollin PE, Ksiazek TG, Sanchez A, Rota PA, Bellini WJ, and Anderson LJ. Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins. J Virol Methods. 2005 Sep;128(1-2):21-8.
- Tripp RA, Oshansky C, and Alvarez R. Cytokines and respiratory syncytial virus infection. Proc Am Thorac Soc. 2005;2(2):147-9. Review.
This page last updated November 8, 2011 .
