Viral Immunology:Tripp Laboratory
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Les Jones
Animal Health Research Center, Room 103
Office:(706) 542-3399
Lab:(706) 542-9862
Research Interests
Respiratory syncytial virus (RSV) is a single-stranded negative sense RNA virus in the Paramyxovirus family that is the primary cause of morbidity and life-threatening lower respiratory tract disease in infants and young children worldwide, as well as an important pathogen of the elderly and immune compromised. Despite over four decades of research, no safe and effective RSV vaccine exists and treatments are limited. In infants and young children, the immune response to RSV is not fully protective, as repeat infections with the same or different strains of RSV are common.
These indications suggest that RSV may modulate or evade the immune response to promote virus infection and replication. Recent studies from our laboratory have shown that RSV can infect and replicate in primary cortical neurons from mice. Since neurons are an immune-privileged site, these studies suggest that RSV may use neurons to establish a state of low-level persistent infection.
The goal of my studies is to use nanotechnology such as the quartz crystal microbalance (QCM) biosensing device and self-assembled sandwich structures consisting of monoclonal antibody-labeled nanoparticles to detect RSV particles in a variety of cells and tissues to elucidate persistence, and provide insights required for development of vaccine and therapeutic intervention strategies to protect public health.
The QCM is basically a mass sensing device with the ability to measure very small mass changes on a quartz crystal resonator in real-time. The QCM is capable of measuring mass changes as small as a fraction of a single layer of atoms. The high sensitivity and the real-time monitoring of mass changes on the sensor crystal make QCM a very attractive technique for a large range of applications. Especially, the development of QCM systems for use in specific recognition of protein ligands by immobilized receptors, immunological reactions, and detection of virus particles, bacteria, mammalian cells, and the formation and prevention of formation of biofilms.
Recent Publications
- Zhang W, Choi Y, Haynes LM, Harcourt JL, Anderson LJ, Jones LP, and Tripp RA. Vaccination to induce antibodies blocking the CX3C-CX3CR1 interaction of respiratory syncytial virus G protein reduces pulmonary inflammation and virus replication in mice. J Virol. 2010 Jan;84(2):1148-57. Epub 2009 Oct 28.
- Mundt E, Gay L, Jones L, Saavedra G, Tompkins SM, and Tripp RA. Replication and pathogenesis associated with H5N1, H5N2, and H5N3 low-pathogenic avian influenza virus infection in chickens and ducks. Arch Virol. 2009;154(8):1241-8. Epub 2009 Jul 3.
- Oshansky CM, Krunkosky TM, Barber J, Jones LP, and Tripp RA. Respiratory syncytial virus proteins modulate suppressors of cytokine signaling 1 and 3 and the type I interferon response to infection by a toll-like receptor pathway. Viral Immunol. 2009 Jun;22(3):147-61.
- Driskell JD, Seto AG, Jones LP, Jokela S, Dluhy RA, Zhao YP, and Tripp RA. Rapid microRNA (miRNA) detection and classification via surface-enhanced Raman spectroscopy (SERS). Biosens Bioelectron. 2008 Dec 1;24(4):923-8. Epub 2008 Aug 6.
- Driskell J, Shanmukh S, Liu YJ, Hennigan S, Jones LP, Zhao YP, Dluhy RA, Krause DC, and Tripp, RA. Infectious agent detection with SERS-active silver nanorod arrays prepared by oblique angle deposition. IEEE, Nanosensors for Defense & Security. 2008, in press.
- Fu J, Jones LP, Tripp RA, and Zhao YP. Au/Si hetero-nanorod-based biosensor for Salmonella detection. Nanotechnology. 2008, in press.
- Shanmukh S, Jones L , Zhao YP, Driskell JD, Tripp RA, and Dluhy RA. Identification and classification of respiratory syncytial virus (RSV) strains by surface enhanced Raman spectroscopy and multivariate statistical techniques. Anal. Bioanal. Chem. 2008 Mar;390(6):1551-5. Epub 2008 Jan 31.
- Tripp RA, Alvarez R, Anderson B, Jones L, Weeks C, and Chen W. Bioconjugated nanoparticle detection of respiratory syncytial virus infection. Int J Nanomedicine. 2007;2(1):117-24.
- Harcourt J, Alvarez R, Jones LP, Henderson C, Anderson LJ, and Tripp RA. Respiratory syncytial virus G protein and G protein CX3C motif adversely affect CX3CR1+ T cell responses. J Immunol. 2006 Feb 1;176(3):1600-8.
- Shanmukh S, Jones L, Driskell J, Zhao Y, Dluhy R, and Tripp RA. Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate. Nano Lett. 2006 Nov;6(11):2630-6.
- Tripp RA, Haynes LM, Moore D, Anderson B, Tamin A, Harcourt BH, Jones LP, Yilla M, Babcock GJ, Greenough T, Ambrosino DM, Alvarez R, Callaway J, Cavitt S, Kamrud K, Alterson H, Smith J, Harcourt JL, Miao C, Razdan R, Comer JA, Rollin PE, Ksiazek TG, Sanchez A, Rota PA, Bellini WJ, and Anderson LJ. Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): identification of neutralizing and antibodies reactive to S, N, M and E viral proteins. J Virol Methods. 2005 Sep;128(1-2):21-8.
- Alvarez R, Jones LP, Seal BS, Kapczynski DR, and Tripp RA. Serological cross-reactivity of members of the Metapneumovirus genus. Virus Res. 2004 Sep 15;105(1):67-73. Erratum in: Virus Res. 2005 Jan;107(1):109.
- Haynes LM, Jones LP, Barskey A, Anderson LJ, Tripp RA. Enhanced disease and pulmonary eosinophilia associated with formalin-inactivated respiratory syncytial virus vaccination are linked to G glycoprotein CX3C-CX3CR1 interaction and expression of substance P. J Virol. 2003 Sep;77(18):9831-44.
- Tripp RA, Dakhama A, Jones LP, Barskey A, Gelfand EW, and Anderson LJ. The G glycoprotein of respiratory syncytial virus depresses respiratory rates through the CX3C motif and substance P. J Virol. 2003 Jun;77(11):6580-4.
This page last updated November 8, 2011 .
