Viral Immunology: Tripp Laboratory
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Jeremy Driskell, PhD
Animal Health Research Center, Room 113
Office: (706) 542-2205
Research Interests
Development of diagnostic methods for rapid and sensitive identification of viruses is essential for the advancement of therapeutic and preventive intervention strategies necessary to protect public health. Current diagnostic methods, including virus isolation, PCR, antigen detection and serology, are time-consuming, cumbersome, or lack the required sensitivity. Thus, there is a critical need to develop novel strategies to overcome the limitations of these traditional methods.
My research focuses on the integration of nanotechnology, spectroscopy, chemistry, and multivariate statistical analysis for the direct detection and classification of intact whole viruses. This method relies on surface-enhanced Raman spectroscopy (SERS) for viral fingerprinting. The strength of a SERS-based biosensor is its abilities to detect extremely low levels of material and to provide virus-specific structural information for identification. Historically, SERS has been limited by the lack of a technology to reproducibly fabricate nanostructured surfaces required for SERS. Recently, oblique angle deposition (OAD) has been developed for the fabrication of nanorod arrays which provides the reproducibility required for SERS-based viral fingerprinting.
OAD-fabricated substrates have been employed to collect SERS spectral libraries for several viruses, including respiratory syncytial virus (RSV), HIV, measles virus, influenza, and rotavirus. Chemometric analysis of the intrinsic SERS spectra collected from these assays facilitates classification of the virus samples, and the results demonstrate that this method has the specificity to differentiate between different strains of a single virus.
Publications
- Driskell JD, Primera-Pedrozo OM, Dluhy RA, Zhao YP, and Tripp RA. Quantitative SERS-Based Analysis of MicroRNA Mixtures. Appl. Spectrosc. 2009;6(10):1107.
- Driskell JD, and Tripp RA. Emerging Technologies in Nanotechnology-based Pathogen Detection. Clinical Microbiology Newsletter 2009;31(18):137.
- Wang G, Driskell JD, Porter MD, and Lipert RJ. Control of Antigen Mass Transport via Capture Substrate Rotation: Binding Kinetics and Implications on Immunoassay Speed and Detection Limit. Anal. Chem. 2009;81:6175.
- Abell JL, Driskell JD, Dluhy RA, Tripp RA, and Zhao YP. Optimization of a Multi-well Array SERS Chip. Proc. SPIE. 2009;7321:1.
- Abell JL, Driskell JD, Dluhy RA, Tripp RA, and Zhao YP. Fabrication and characterization of a multiwell array SERS chip with biological applications. Biosens Bioelectron. 2009 Aug 15;24(12):3663-70. Epub 2009 Jun 7.
- Driskell JD, Seto AG, Jones LP, Jokela S, Dluhy RA, Zhao YP, and Tripp RA. Rapid MicroRNA (miRNA) Detection and Classification via Surface-Enhanced Raman Spectroscopy (SERS). Biosens. Bioelectron. 2008;24:923.
- Driskell JD, Shanmukh S, Liu Y, Chaney SB, Tang XJ, Zhao YP, and Dluhy RA. The use of aligned silver nanorod arrays prepared by oblique angle vapor deposition as SERS substrates. J. Phys. Chem. C. 2008;112:895.
- Shanmukh S, Jones L, Zhao YP, Driskell JD, Tripp RA, and Dluhy RA. Identification and classification of respiratory syncytial virus (RSV) strains by surface enhanced Raman spectroscopy and multivariate statistical techniques. Anal. Bioanal. Chem. 2008 Mar;390(6):1551-5. Epub 2008 Jan 31.
- Driskell JD, Shanmukh S, Liu YJ, Hennigan S, Jones L, Zhao YP, Dluhy RA, Krause DC, and Tripp RA. Infectious agent detection with SERS-active silver nanorod arrays prepared by oblique angle deposition. IEEE Sens. J. 2008, 8(6), 863.
- Driskell JD, Uhlenkamp JM, Lipert RJ, and Porter MD. Surface-enhanced Raman scattering immunoassays using a rotated capture substrate. Anal. Chem. 2007 Jun 1;79(11):4141-8. Epub 2007 May 8.
- Driskell JD, Kwarta KM, Lipert RJ, Porter MD, Neill JD, and Ridpath JF. Control of antigen mass transfer via capture substrate rotation: an absolute method for the determination of antigen concentration and reduction of immunoassay incubation times. J. Virol. Meth. 2006 Dec;138(1-2):160-9. Epub 2006 Oct 10.
- Driskell JD, Lipert RJ, and Porter MD. Labeled gold nanoparticles immobilized at smooth metallic substrates: systematic investigation of surface plasmon resonance and surface-enhanced Raman scattering. J. Phys. Chem. B. 2006 Sep 7;110(35):17444-51.
- Shanmukh S, Jones L, Driskell J, Zhao Y, Dluhy R, and Tripp R.A. Rapid and sensitive detection of respiratory virus molecular signatures using a silver nanorod array SERS substrate. Nano Lett. 2006 Nov;6(11):2630-6.
- Porter MD, Driskell JD, Kwarta KM, Lipert RJ, Neill JD, and Ridpath JF. Detection of viruses: atomic force microscopy and surface-enhanced Raman spectroscopy. Developments in Biologicals 2006;126:31-9; discussion 323.
- Driskell JD, Kwarta KM, Lipert RJ, Porter MD, Neill JD, and Ridpath JF. Low-level detection of viral pathogens by a surface-enhanced Raman scattering based immunoassay. Anal. Chem. 2005 Oct 1;77(19):6147-54.
- Grubisha DS, Lipert RJ, Park HY, Driskell JD, and Porter MD. Femtomolar detection of prostate-specific antigen: an immunoassay based on surface-enhanced Raman scattering and immunogold labels. Anal. Chem. 2003 Nov 1;75(21):5936-43.
Book Chapters
- Kenseth JR, Kwarta KM, Driskell JD, Porter MD, Neill JD, Ridpath JF. Strategies in the use of atomic force microscopy as a multiplexed readout tool of chip-scale protein motifs. Combinatorial Materials Science. S. Mallapragada and B. Narasimhan, Eds.; Wiley, 2007.
- Park HY, Driskell JD, Kwarta KM, Lipert RJ, Porter MD, Schoen C, Neill JD, Ridpath JF. Ultrasensitive immunoassays based on surface-enhanced Raman scattering by immunogold labels” Surface-Enhanced Raman Scattering – Physics and Applications. K. Kneipp, H. Kneipp, and M. Moskovits, Eds.; Springer, 2006.
- Driskell JD, Shanmukh S, Liu Y, Chaney S, Hennigan S, Jones L, Krause D, Tripp RA, Zhao YP, Dluhy RA. Novel nanoarray SERS substrates used for high sensitivity virus biosensing and classification. Nanoscience and Nanotechnology for Chembio Defense. Ramanathan, Ed.; ACS, 2007 in press.
This page last updated March 10, 2010 .
